{"id":3303,"date":"2020-11-13T17:09:00","date_gmt":"2020-11-13T16:09:00","guid":{"rendered":"https:\/\/fhu-premimpact.org\/?post_type=publication&#038;p=3303"},"modified":"2021-02-06T17:12:29","modified_gmt":"2021-02-06T16:12:29","slug":"characterization-of-non-toxigenic-clostridioides-difficile-strains-isolated-from-preterm-neonates-and-in-vivo-study-of-their-protective-effect","status":"publish","type":"publication","link":"https:\/\/fhu-premimpact.org\/en\/publications\/characterization-of-non-toxigenic-clostridioides-difficile-strains-isolated-from-preterm-neonates-and-in-vivo-study-of-their-protective-effect\/","title":{"rendered":"Characterization of Non-Toxigenic Clostridioides difficile Strains Isolated from Preterm Neonates and In Vivo Study of Their Protective Effect."},"content":{"rendered":"\n<h4 class=\"wp-block-heading\">Abstract<\/h4>\n\n\n\n<p>In a previous monocentric study in preterm neonates (PN), we described a high&nbsp;<em>Clostridioides difficile<\/em>&nbsp;colonization rate (74%) with two uncommon non-toxigenic strains (NTCD) belonging to PCR-ribotype (RT) (CE)847 and (CE)032. To determine the extent of carriage of both NTCD in other spatio-temporal settings, strains isolated in PN stools from two multicenter cohorts were characterized by PCR-ribotyping, MLVA and MLST. We also evaluated the protective role of two NTCD from these RT against&nbsp;<em>C. difficile<\/em>&nbsp;infection in a hamster caecitis model. Animals were administered either each NTCD alone (n = 7), or followed by a 027 strain (n = 9). A control group received only the 027 strain (n = 8). Clinical activity and colonization by&nbsp;<em>C. difficile<\/em>&nbsp;in stools were monitored daily until death or sacrifice at D20. We isolated 18 RT(CE)032 (ST-83) strains and 2 RT(CE)847 (ST-26) strains among 247 PN from both cohorts. Within each RT, strains were genetically related. The survival rate was significantly increased when animals received a RT(CE)847 or (CE)032 strain before the 027 strain (4\/9 deaths,&nbsp;<em>p<\/em>&nbsp;= 0.029; 1\/9 death,&nbsp;<em>p<\/em>&nbsp;= 0.0004, respectively). We describe two predominant uncommon NTCD strains, in a PN population from different healthcare facilities. Both NTCD provide a potential protection against&nbsp;<em>C. difficile<\/em>&nbsp;infection.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>J Clin Med. 2020 Nov 13;9(11):3650. doi: 10.3390\/jcm9113650. PMID: 33202811; PMCID: PMC7696784.<\/p>\n","protected":false},"featured_media":0,"template":"","class_list":["post-3303","publication","type-publication","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Characterization of Non-Toxigenic Clostridioides difficile Strains Isolated from Preterm Neonates and In Vivo Study of Their Protective Effect. - FHU Prem&#039;IMPACT<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/fhu-premimpact.org\/en\/publications\/characterization-of-non-toxigenic-clostridioides-difficile-strains-isolated-from-preterm-neonates-and-in-vivo-study-of-their-protective-effect\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Characterization of Non-Toxigenic Clostridioides difficile Strains Isolated from Preterm Neonates and In Vivo Study of Their Protective Effect. - FHU Prem&#039;IMPACT\" \/>\n<meta property=\"og:description\" content=\"J Clin Med. 2020 Nov 13;9(11):3650. doi: 10.3390\/jcm9113650. 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